ScienCell 0203 台盼蓝TB Trypan Blue (0.4%),50 ml,产品说明: 台盼蓝(C34H24N6Na4O14S4,MW 960.81)溶液是一种用于区分活细胞和非活细胞的染色剂。非活性细胞将吸收染料并呈现蓝色,而活性细胞将排除染料。细胞应在计数前置于缓冲盐水中的单细胞悬浮液中。由于台盼蓝对血清蛋白的亲和力高于细胞蛋白,因此将细胞悬浮在含有血清的培养基中会产生暗背景。
Trypan Blue (C34H24N6Na4O14S4, MW 960.81) solution is a stain used to distinguish viable from nonviable cells. Nonviable cells will absorb the dye and appear blue, while viable cells will exclude the dye. Cells should be in a single cell suspension in buffered saline before counting. Since trypan blue has a higher affinity for serum protein than for cellular proteins, suspending the cells in medium containing serum will generate a dark background.