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- 技术资料
- 库存：
99
- 英文名：
ScienCell Mycoplasma PCR Detection Kit
- 供应商：
北京诺博莱德科技有限公司
8208 ScienCell 支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit
ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。
北京诺博莱德作为ScienCell品牌的特约经销商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。
8208 ScienCell 支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit，产品简介：
产品品牌; ScienCell
产品货号：8208
产品名称：8208 ScienCell 支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit
产品规格：100 Tests
我们提供较快和较敏感的细胞分析检测，用于高通量的生物医学研究、药物筛选和诊断领域。基于细胞的检测是必要的、有用的临床实验室研究工具。ScienCell研究实验室已经开发出多种的细胞检测试剂盒，用于不同领域的研究，包括代谢、氧化应激、酶活性分析等。
8208 ScienCell 支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit,产品说明：
支原体是最小的自我复制的原核生物，是原代细胞和细胞系的常见污染物。支原体污染会影响细胞生长、形态和代谢。支原体由于其体积小而能够通过0.2μm过滤器。此外，与细菌和真菌不同，支原体污染对常用抗生素具有抗药性，无法通过视觉识别。由于检测支原体的困难，应定期筛查细胞以控制支原体污染。常规筛选方法包括微生物培养、荧光 DNA 染色和生化检测方法，通常耗时且缺乏灵敏度。因此，基于聚合酶链反应（PCR）的方法已被开发出来，作为低水平支原体感染的快速便捷检测，同时提供高灵敏度和特异性。支原体16S rRNA序列的比对研究揭示了高度保守序列的存在。靶向这些序列的引物允许支原体DNA片段的特异性扩增。ScienCell™ 支原体 PCR 检测试剂盒是一种基于 16S rRNA 的 PCR 检测试剂盒，由 2×PCR 预混液（包括 PCR 缓冲液、DNA 聚合酶和 dNTP）、支原体引物组和阳性对照组成，阳性对照是发酵分枝杆菌的非感染性基因组 DNA （gDNA）。属特异性引物集可识别占污染 98% 的五种典型污染支原体物种（即猪鼻支原体、阿吉尼尼支原体、口服支原体、发酵支原体和莱德拉维支原体），以及占剩余 2% 污染的脲原体、螺旋体和无胆原体属的成员。真核生物和细菌DNA不会被该试剂盒扩增。预期波段的大小为 280 bp。
Mycoplasma, the smallest and self-replicating prokaryotes, are common contaminants of primary cells and cell lines. Mycoplasma contamination can affect cell growth, morphology and metabolism. Mycoplasma are able to pass through 0.2 µm filters due to their small size. Furthermore, unlike bacteria and fungi, mycoplasma contamination is resistant to commonly used antibiotics, and can not be identified visually. Due to the difficulties in detecting mycoplasma, cells should be regularly screened to control mycoplasma contamination. Conventional screening methods include microbiological culture, fluorescent DNA staining, and biochemical detection methods, and are typically time consuming and lack sensitivity. As a result, polymerase chain reaction (PCR) based methods have been developed as a quick and convenient detection of low level mycoplasma infection, while providing high sensitivity and specificity. Alignment studies of mycoplasma 16S rRNA sequences revealed the existence of highly conserved sequences. Primers targeting these sequences allow for the specific amplification of the mycoplasma DNA fragment. ScienCell™ Mycoplasma PCR Detection Kit is a 16S rRNA-based PCR assay consisting of 2×PCR master mix (which includes PCR buffer, DNA polymerase and dNTPs), mycoplasma primer set, and a positive control, which is noninfectious genomic DNA (gDNA) of M. fermentans. The genus-specific primer set recognizes the five typical contaminating mycoplasma species (i.e., M. hyorhinis, M. arginini, M. orale, M. fermentans, and A. laidlawi), which account for 98% of contamination, as well as members of the genera Ureaplasma, Spiroplasma, and Acholeplasma, which account for the remaining 2% of contamination. Eukaryotic and bacterial DNA is not amplified by this kit. The size of the expected band is 280 bp.
ScienCell试剂盒产品订购信息：
3D-4110 3D肾小管上皮细胞形成试剂盒 3D-RTF，3D Renal Tubule Formation Kit Kit
0223 茜素红S染色试剂盒 ARed，Alizarin Red S Staining Kit 100ml
0833 人胚层鉴定试剂盒 HGL PCR，Human Germ Layer Detection Kit 50 reactions
0843 油红O染色试剂盒 OEed，Oil Red O Staining Kit 100 ml/bottle - 2 bottles
0853 人干细胞多能性检测试剂盒 HSC PCR，Human Stem Cell Pluripotency Detection Kit 50 reactions
3518 总谷*甘*检测试剂盒 TGA， Total Glutathione Assay 100 tests
8008 ScienCellI型胶原细胞粘附检测试剂盒，Collagen I Cell Adhesion AssayKit 48 tests
8018 纤维粘连细胞粘附检测试剂盒，Fibronectin Cell Adhesion Assay 48 tests
8028 MTT细胞活力和增殖检测试剂盒，MTT Cell Viability & Proliferation Assay 1000 tests in 96-well plate
8038 WST-1细胞活力和增值检测试剂盒，WST-1 Cell Viability & Proliferation Assay Kit
8058 细胞衰老检测试剂盒，Cell Senescence Assay 50 tests/35 mm plate
8068 β-半乳糖苷酶比色检测试剂盒 Galc，Beta-Galactosidase Colorimetric Assay 50 tests/35 mm plate
8078 LDH细胞*性检测试剂盒 LDH，LDH Cytotoxicity Assay 500 tests
8088 TUNEL 色标记细胞凋亡检测试剂盒，Colorimetric TUNEL Apoptosis Assay 50 tests
8098 一氧**比色法检测试剂盒 NO，Colorimetric Nitric Oxide Assay 250 tests
8108 磷酸酶检测试剂盒 pNPP，Phosphatase Assay 500 tests
8118 孔雀绿磷酸盐检测试剂盒 MGPA，Malachite Green Phosphate Assay 2500 tests
8128 比色钙法分析试剂盒 CCA，Colorimetric Calcium Assay 250 tests
8138 活/死细胞染色试剂盒，Live/Dead Cell Staining Kit 1000 tests
8148 比色法GAPDH分析试剂盒 GAPDH，Colorimetric GAPDH Assay 100 tests
8158 体外管腔形成分析试剂盒，In Vitro Tube Formation Assay 50 tests
8168 总抗氧化能力检测试剂盒 TACATotal， Antioxidant Capacity Assay 100 tests
8178 胶原 I-3D凝胶试剂盒，Collagen-based Culturing Kits 100 mg
8198 超氧化物歧化酶分析试剂盒 SOD，Superoxide Dismutase Assay 100 tests
8208 支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit 100 Tests
8218 过*化*酶活性分析试剂盒 CAT， Catalase Activity Assay 100 tests
8228 细胞凋亡蛋白酶-3分析试剂盒， Caspase-3 Assay 100 tests
8238 谷*甘*过氧化*酶检测试剂盒 GPx， Glutathione Peroxidase Assay 100 tests
8258 碱性磷酸酶活力检测试剂盒 ALP， Alkaline Phosphatase Activity Assay 500 tests
8268 线粒体分离试剂盒 MITOISO， Mitochondria Isolation Kit 100 tests
8278 细胞色素C氧化酶检测试剂盒 COX， Cytochrome C Oxidase Assay 100 tests
8288 碱性磷酸酶染色试剂盒（红）ALPr， Alkaline Phosphatase Staining Assay (Red) 500 tests
8298 人间充质干细胞脂肪形成检测试剂盒 HMSC-A PCR， Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
8308 L-乳酸盐检测试剂盒 LAC， L-Lactate Assay 100 tests
8318 柠檬酸合成酶检测试剂盒 CS， Citrate Synthase Assay 50 tests in cuvette or 250 tests in 96-well plate
8328 人间充质干细胞软骨形成检测试剂盒 HMSC-C PCR， Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
8338 谷*酸检测试剂盒 GLU， Glutamate Assay 100 tests
8358 谷*酸脱*酶检测试剂盒 GDH， Glutamate Dehydrogenase Assay 100 tests
8368 NAD/NADH检测试剂盒 NAD， NAD/NADH Assay 100 tests
8378 阿尔新蓝染色试剂盒 ABlue， Alcian Blue Staining Kit 100 tests
8388 储液瓶 传统风格 125ml 45mm颈尺寸 带盖 灭菌， Costar® 125mL Traditional Style Polystyrene Storage Bottles with 45mm Caps 100 tests
8398 葡萄糖6磷酸酶检测试剂盒 G6P ， Glucose-6-phosphate Assay 100 tests
8408 已糖激酶检测试剂盒 HK ， Hexokinase Assay 100 tests
8418 葡萄糖检测试剂盒 GL， Glucose Assay 100 tests
8428 葡糖6磷酸脱氢酶检测试剂盒 G6PDH， Glucose-6-phosphate Dehydrogenase Assay 100 tests
8438 尿酸检测试剂盒 US， Uric Acid Assay 100 tests
8448 铁检测试剂盒， Iron Assay 100 tests
8458 黄*呤氧化酶检测试剂盒 XO， Xanthine Oxidase Assay 100 tests
8468 甘油检测试剂盒 GLY， Glycerol Assay 100 tests
8478 **酸转氨酶检测试剂盒 ALT， Alanine Transaminase Assay 100 tests
8498 甘油三酸酯检测试剂盒 TG， Triglyceride Assay 100 tests
8508 3-甘油磷酸脱氢酶检测试剂盒 GPDH， Glycerol-3-phosphate Dehydrogenase Assay 100 tests
8528 *检测试剂盒 AA， Ammonia Assay 100 tests
8548 谷*甘*S转移酶检测试剂盒 GST， Glutathione S-transferases Assay 100 tests
8558 GSH/GSSG比例检测试剂盒 GSH/GSSG， GSH/GSSG Ratio Assay 100 tests
8568 乙醇脱氢酶活性检测试剂盒 ADH， Alcohol Dehydrogenase Assay 100 tests
8578 醛胶氢酶检测试剂盒 ALDH， Aldehyde Dehydrogenase Assay 100 tests
8588 胆碱检测试剂盒 Cho， Choline Assay 100 tests
8598 磷酯酶D检测试剂盒 PLD， Phospholipase D Assay 100 tests
8608 天冬氨酸转氨酶检测试剂盒 AST， Aspartate Transaminase Assay 100 tests
8618 肌酸激酶检测试剂盒 CK， Creatine Kinase Assay 100 tests
8628 **酸激酶检测试剂盒 PK， Pyruvate Kinase Assay 100 tests
8638 苹果酸脱氢酶检测试剂盒 MDH， Malate Dehydrogenase Assay 100 tests
8648 苹果酸盐检测试剂盒 Mal， Malate Assay 100 tests
8658 **酸脱氢酶检测试剂盒 PDH， Pyruvate dehydrogenase Assay 100 tests
8668 比色法组蛋白乙酰转移酶活性测定， Colorimetric Histone Acetyltransferase Activity Assay 100 Tests in 96-well plate
8678 茜素红S染色量化学分析试剂盒 ARS， Alizarin Red S Staining Quantification Assay 100 tests
8688 3D胶原I基质试剂盒， 3D Collagen I Matrix Kit 1盒
8698 3D基础嵌入式管腔形成试剂盒， 3D Basic Embedded Tubule Formation Kit 1盒
8708 3D嵌入式管腔形成试剂盒， 3D Embedded Tubule Formation Kit 1盒
8718 3D网络结构检测试剂盒， 3D Network Formation Assay Kit 1盒
8728 3D内皮细胞-外膜细胞共培养试剂盒， 3D Endothelial–Pericyte Coculturing Kit 1盒
8808 3D凝胶染色准备试剂盒， 3D Gel Staining Prep Kit 5 x 20ml
8888 比色法组蛋白去乙酰化酶活性检测试剂盒HDAC， Colorimetric Histone Deacetylase Activity Assay 100 tests
8898 比色法胰蛋白酶活性检测试剂盒TRYP， Colorimetric Trypsin Activity Assay 100 tests
8908 人端粒长度定量qPCR分析试剂盒（相对定量）， Relative Human Telomere Length Quantification qPCR Assay Kit 100 tests
8918 人端粒长度定量qPCR分析试剂盒（绝对定量）， Absolute Human Telomere Length Quantification qPCR Assay Kit 100 reactions
8928 端粒酶活性定量qPCR检测试剂盒，Telomerase Activity Quantification qPCR Assay Kit 100 reactions
8938 人线粒体DNA拷贝数定量qPCR分析试剂盒（相对定量）RHMQ，Relative Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit 100 reactions
8948 人线粒体DNA拷贝数定量qPCR分析试剂盒（绝对定量）AHMQ，Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit 100 reactions
8958 人端粒长度和线粒体DNA拷贝数双重分析定量qPCR分析试剂盒（绝对定量）AHDQ，Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit 100 reactions
8988 HeLa细胞污染检测试剂盒ULCC，HeLa Cell Contamination Detection Kit 100 reactions
M8908 小鼠端粒长度定量qPCR分析试剂盒（相对定量）RMTLQ，Relative Mouse Telomere Length Quantification qPCR Assay Kit 100 reactions
M8918 小鼠端粒长度定量qPCR分析试剂盒（绝对定量）AMTLQ，Absolute Mouse Telomere Length Quantification qPCR Assay Kit 100 reactions
P8918 猪端粒长度定量qPCR分析试剂盒（绝对定量）APTLQ，Absolute Pig Telomere Length Quantification qPCR Assay Kit 100 reactions
R8908 大鼠鼠端粒长度定量qPCR分析试剂盒（相对定量）RRTLQ，Relative Rat Telomere Length Quantification qPCR Assay Kit 100 reactions
R8918 大鼠端粒长度定量qPCR分析试剂盒（绝对定量）ARTLQ，Absolute Rat Telomere Length Quantification qPCR Assay Kit 100 reactions
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ScienCell8208支原体PCR检测试剂盒，Mycoplasma PCR Detection Kit
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