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文献和实验Extraction of DNA using DNAzol Reagent
without additional purification for applications such as Southern analysis, dot blot hybridization, molecular cloning, and polymerase chain reaction (PCR). 主要试剂 DNAzol® Reagent 100% ethanol 75% ethanol 8 N NaOH 实验步骤 1 Lysis
Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
, filtrate; add several drops of bromic water to be oxidated to pale yellow while the filtrate is green. Keep the filtrate in brown flask in darkroom. Titrate with calibration NaOH before using to ascertain the acidity of the reagent, the indicator
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