In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. 4-Di-10-ASP (1 μM), DOPC (HY-113424A) (10 mM), and DOPG (HY-142980) (1 mM) is dissolved in methanol/chloroform (25 L, 1:2 v/v). 2. The solution is allowed to dry overnight under vacuum to obtain lamellar lipid films, which in turn are hydrated with the transcription/translation solution (25 L) for three hours at 37℃. 3. An aliquot (10 L) of the solution thus prepared is placed on a glass slide and sealed by a cover glass. 4. The sample is immediately observed with a confocal laser-scanning microscope, an argon laser (488 nm) is employed to excite the 4-Di-10-ASP.