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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt)
- 库存:
10
- 供应商:
TargetMol
- CAS号:
206443-06-3
- 规格:
10 mg/1 mg/5 mg
| 规格: | 10 mg | 产品价格: | ¥5330.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥1640.0 |
| 规格: | 5 mg | 产品价格: | ¥2950.0 |
Product Introduction
Bioactivity
英文名:4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt)
描述:4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
methods developed to examine long-range chromosomal interactions in vivo, see Simonis et al. (2007). For additional details on the 6C method presented here, see Tiwari et al. (2008). MATERIALS Reagents 5-Bromo-4-chloro-3-indoyl-β-D
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
to an equal volume of Assay 2X Buffer, which contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl
Deglycosylation of Glycoproteins Using Endoglycosidases
even after denaturation in SDS. A typical test reaction on human transferrin is described in the protocol below. Materials: Transferrin Sodium Dodecyl sulfate Nonidet P-40 Gel electrophoresis apparatus
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