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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Human respiratory syncytial virus A (strain A2) & Bovine respiratory syncytial virus (strain Rb94) (BRS)
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, IF, RIA, WB
- 抗体英文名:
Anti-HRSV-A/BRSV F/Fusion glycoprotein F0 Antibody (B4-1)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2300.0 |
| 规格: | 1mg | 产品价格: | ¥11160.0 |
| 产品名 | Anti-HRSV-A/BRSV F/Fusion glycoprotein F0 Antibody (B4-1) |
| 货号 | RVV02802 |
| 靶标 | F, Fusion glycoprotein F0, Protein F |
| 克隆号 | B4-1 |
| 克隆类型 | Monoclonal |
| 同种型 | IgG1, kappa |
| 宿主 | Human |
| 种属反应性 | Human respiratory syncytial virus A (strain A2) & Bovine respiratory syncytial virus (strain Rb94) (BRS) |
| 应用 | ELISA, IF, RIA, WB |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |

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文献和实验b) Determine and record A280 and A492. c) Calculate protein concentration: protein (mg/mL) = (A280- (A492 x 0.35))/1.4 d) Calculate moles of protein: = protein(mg/mL)/1.5x10^5 e) Calculate moles of FITC: = A492/0.69x10^5 f) Determine F
. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0 b) Determine and record A280 and A492. c) Calculate
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
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