E1014-5KU*SIGMABenzonase®核酸酶

说明

一般描述

Benzonase®全能核酸酶是一种通过高效基因工程法从dacome中获得的核酸内切酶。这种具有两个必需二硫键可在宽泛的操作条件下攻击并降解所有形式的DNA和RNA（单链、双链、线性和环化）。它能将核酸完全消化成长度为3到5个碱基的5′-单磷酸末端寡核苷酸，使其成为从重组蛋白中去除核酸的理想工具，并用于需要完全消化核酸的应用。除了降低蛋白质提取物的粘度和并防止细胞结团之外，使用Benzonase®全能核酸酶预处理蛋白质样本可以消除任何结合的核酸，进而显著提高2D凝胶电泳的分辨率。这种多功能酶可消化天然或热变性的DNA和RNA，其酶活性的最佳pH值为8.0-9.2。Benzonase®全能核酸酶能够去除核酸并提高蛋白质样本的纯度和质量。

应用

Benzonase® Nuclease has been used: as a component in ice-cold lysis buffer C to digest DNA and  RNA to facilitate the complete release of all nuclear proteins[1] in the immunoprecipitation step to release protein complexes from the nucleoplasm and chromatin[2]as a supplement in RIPA to fractionate SHSY5Y cells for immunoprecipitation[3] to remove residual nucleic acids from the aortic roots in decellularization method[4]

用于去除蛋白样品中的核酸。

生化/生理作用

Benzonase® Nuclease can completely digest nucleic acids into 5′-monophosphate terminated oligonucleotides of 3 to 5 bases in length, making it the ideal tool for removing nucleic acids from recombinant proteins and for applications that require complete digestion of nucleic acids. In addition to reducing viscosity in protein extracts and preventing cell clumping, pretreatment of protein samples with Benzonase® nuclease can significantly improve their resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. This versatile enzyme can digest both native or heat-denatured DNA and RNA, with its optimum pH for enzyme activity found to be 8.0-9.2. Benzonase® nuclease is effective at removing host DNA from microbiome samples. In many cases, microbiome samples (such as saliva or skin) will have a high percentage of host DNA that interferes with downstream results. Our experts show that the reduction of host DNA lowers the cost of sequencing while increasing and improving the data. Experimental data is shown in the technical article - Benzonase® Nuclease for Microbiome Workflows

消化天然或热变性的DNA和RNA。

特点和优势

微生物组样本中宿主DNA的损耗

多种工作流程中核酸的有效消化

蛋白质提取过程中粘度的降低

单位定义

在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。

外形

在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。