Sephadex® G-25*SIGMA*G25150-50

说明

一般描述

Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

应用

分馏范围（MW）

右旋糖酐：100 - 5,000

球状蛋白质1,000 - 5,000

Sephadex® G-25已用于：

蛋白酶脱盐[1]

在放射性碘标记oTP-1的表征和受体分析确认过程中，用色谱柱从游离碘中分离放射性碘标记的oTP-1[2]

Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

生化/生理作用

Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

特点和优势

Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.

High recovery rate with minimal sample dilution.

Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.

Specifically designed as a BioProcess resin for industrial applications.

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