LightShift (R) EMSA Optimizati

Introduction to the EMSA (Gel Shift) Technique

Chemiluminescent EMSA Kit, Product # 20148 ) and a kit to facilitate labeling DNA with biotin (Biotin 3´ End DNA Labeling Kit, Product # 89818 ). The LightShift EMSA Kit utilizes Pierce’s patented SuperSignal Chemiluminescent Detection Technology to offer detection

Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization

for normal growth and development of higher eukaryotes. Epigenetic control is an intricate interplay between various molecular mechanisms, e.g. DNA methylation and histone modifications (reviewed in [1-4]). Whereas DNA methylation has been studied in great

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis