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文献和实验SequalPrep Long PCR Kit with dNTPs
0.4 μl -- SequalPrep™ 10X Enhancer A or B 1–2 μl* 0.5X–1X SequalPrep™ Long Polymerase
Southern Hybridization Experiment Kit
of the genome from which the probe sequence is taken. Southern hybridization analysis requires several steps. Consult this manual to plan your laboratory sessions. Some of the introduction to this kit was excerpted with permission from Recombinant DNA
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
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