Store at -20°C. Avoid repeated freezing and thawing
形态:
液体
亚型:
电议
免疫原:
电议
规格:
100 μl
货号: 26474
基因符号: H-Ras; K-Ras; N-Ras
描述: Anti-RAS(G13R) Mouse Monoclonal Antibody
背景介绍: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
免疫原: A synthetic peptide from the internal region of Ras, which includes the mutation of G13R, human origin
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性: Recognizes G13R mutant, but not wild-type RAS of vertebrates.
储存条件: Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:
Western blot analysis of recombinant HRAS(G13R) and wildtype proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with Anti-RAS(G13R) monoclonal antibody (Cat. #26474). 免疫荧光:
Immunofluorescence of cells expressing Ras proteins with Anti-RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13V) plasmid, pCDNA3-GFP-Ras (G13A) plasmid, or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with Anti-RAS(G13R) monoclonal antibody (Cat. #26474).
文献刊登在世界前 50 名期刊上 (谷歌学术搜索) 级别 期刊名称 1. Nature 7. Nature Communications 8. Cell 13. Proceedings of the National Academy of Sciences 18. Nucleic Acids Research 20. Nature Medicine 25. ACS Nano 29. Scientific Reports 33. PLoS ONE 34. Science Advances 37. Nature Genetics 41. International Journal of Molecular Sciences 49. Frontiers in Immunology 文献按产品排名