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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
520 Thiol Quantitation Assay Kit *Fluorimetric*
- CAS号:
520 Thiol Quantitation Assay Kit *Fluorimetric*
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
-20°C
- 规格:
1 kit
520 硫醇定量检测试剂盒为硫醇浓度的测量提供了一种方便、高灵敏度的荧光检测方法。试剂盒中提供的新型试剂与硫醇反应后会产生荧光,在激发/发射=490/520 nm 时可轻松检测到该荧光。该测定的线性范围为 0.04-160 µM 硫醇。硫醇是一种含有巯基的化合物,是强大的还原剂,能够充当抗氧化剂、酶辅因子和神经调节剂。
Note: Warm all kit components until thawed to room temperature before starting the experiments.
Thiol detection reagent solution: Reconstitute one vial of Thiol detection reagent (Component A) in 100 uL of DMSO (Component D). Mix the reagents thoroughly. The stock solution is good for 1 week if stored at -20℃.
Dilute the stock solution of Thiol detection reagent 100 fold in assay buffer (Component C) according to Table 1. For each experiment, prepare fresh detection solution.
Prepare GSH standard solution: Dilute the GSH stock solution 1:50 to 200 um in assay buffer (Component C). Do 2-fold serial dilutions to get concentrations of 100, 50, 25, 12.5, 6.3, and 3.2 uM. Include a blank control.
Add 1-10 uL of test sample into microplate wells.
Note 1: Use assay buffer (Component C) to dilute test samples
Note 2: If the samples are diluted in buffers containing substances that may affect assay performance, test the same amount of that buffer with glutathione standards.
Set up the GSH standard: Add 10 uL serially diluted GSH standard solution (from Step 1.2) to the wells.
Bring the total volume of all controls and samples to 10 uL.
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文献和实验双向凝胶电泳-飞行时间质谱法分析人肺鳞癌细胞HCL-H520蛋白质组成份
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一、如何翻译出好标题?1、拆分关键成纤维细胞源性外泌体-miR- 520b 作为潜在的胰腺癌治疗手段2、理清关键词成纤维细胞源性外泌体-miR- 520b 作为潜在的胰腺癌治疗手段纤维细胞能够释放外泌体,外泌体中的 miR- 520b 能够影响胰腺癌细胞。3、百度学术模仿提取核心关键词核心关键词搜索影响因子查询网站http://www.letpub.com.cn/index.php?page = journalapp&view = search1、输入核心关键词2、选择「SCI 索引」条目
jerome520 我现在急做一批样本的microRNA检测(只检测一个指标),用茎环法,想要送去公司做。康成生物高手们有听说过吗?还有其他代做的公司推荐吗?谢谢!!! zaoyii 我知道广州伯信生物技术有限公司可以做,技术上也比较成熟,这是网站http://www.biosensechina.com/,你可以去看看或者打电话去问问:020-28069086 jerome520
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