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- 详细信息
- 询价记录
- 技术资料
- 库存:
大量
- 英文名:
520 SARS-CoV-2 Papain-like Protease/ De-ubiquitinase Activity Assay Kit
- CAS号:
520 SARS-CoV-2 Papain-like Protease/ De-ubiquitinase Activity Assay Kit
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
-80°C
- 规格:
1 kit
SARS-CoV-2 木瓜蛋白酶 (PLpro) 活性检测试剂盒 *荧光法*、520 SARS-CoV-2 Papain-like Protease/ De-ubiquitinase Activity Assay Kit详细如下:
520 SARS-CoV-2 类木瓜蛋白酶/去泛素酶活性检测试剂盒采用荧光肽底物检测去泛素化活性。当活性类木瓜蛋白酶切割底物时,会导致激发/发射 = 490 nm/520 nm 处的亮绿色荧光增加。该测定可检测样品中低至 7.8 ng/mL 的活性木瓜蛋白酶样蛋白酶。该试剂盒可用于检测纯化酶制剂、生物样品中的酶活性,也可用于化合物筛选。
应用:蛋白酶活性、FRET
检测方法:荧光灯
主持人:大肠杆菌
1. Prepare SARS-CoV-2 Papain-like protease containing biological samples.
1.1 Prepare sample from cell culture medium:
• Collect medium from culture.
• Spin the medium sample for 10-15 min. at 1,000X g, 4C.
• Collect the supernatant and store at -70C until use.
1.2 Prepare cell lysates:
• Cells are collected by centrifugation at 500 X g for 10 min.
• Add an appropriate amount of cold lysate buffer to cell pellet. Collect the cell
suspension to a microcentrifuge tube.
• Incubate the cell suspension on ice for at least 10 min.
• Pipette the cell suspension up and down for 5 times.
• Centrifuge the cell suspension for 5 min. at 10,000 X g, 4°C. Collect the supernatant
and store at -70°C until use
520 SARS-CoV-2 类木瓜蛋白酶/去泛素酶活性检测试剂盒采用荧光肽底物检测去泛素化活性。当活性类木瓜蛋白酶切割底物时,会导致激发/发射 = 490 nm/520 nm 处的亮绿色荧光增加。该测定可检测样品中低至 7.8 ng/mL 的活性木瓜蛋白酶样蛋白酶。该试剂盒可用于检测纯化酶制剂、生物样品中的酶活性,也可用于化合物筛选。
应用:蛋白酶活性、FRET
检测方法:荧光灯
主持人:大肠杆菌
1. Prepare SARS-CoV-2 Papain-like protease containing biological samples.
1.1 Prepare sample from cell culture medium:
• Collect medium from culture.
• Spin the medium sample for 10-15 min. at 1,000X g, 4C.
• Collect the supernatant and store at -70C until use.
1.2 Prepare cell lysates:
• Cells are collected by centrifugation at 500 X g for 10 min.
• Add an appropriate amount of cold lysate buffer to cell pellet. Collect the cell
suspension to a microcentrifuge tube.
• Incubate the cell suspension on ice for at least 10 min.
• Pipette the cell suspension up and down for 5 times.
• Centrifuge the cell suspension for 5 min. at 10,000 X g, 4°C. Collect the supernatant
and store at -70°C until use
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SARS-CoV-2 木瓜蛋白酶 (PLpro) 活性检测试剂盒 *荧光法*
¥7154










