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- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
Rh110 Elastase Assay Kit *Fluorimetric*
- CAS号:
Rh110 Elastase Assay Kit *Fluorimetric*
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
-20°C
- 规格:
1 kit
Rh110 弹性蛋白酶检测试剂盒 荧光法、Rh110 Elastase Assay Kit *Fluorimetric*详细如下:
Rh110 弹性蛋白酶检测试剂盒使用荧光肽底物检测弹性蛋白酶活性。弹性蛋白酶裂解后,肽释放出具有亮绿色荧光的 Rh110 荧光团,可在 Ex/Em=496 /520 nm 处检测到。荧光强度的增加与酶活性成正比。该试剂盒不需要任何分离步骤,可用于连续测量弹性蛋白酶的动力学。Rh110 的更长波长光谱和更高消光系数提供更高的灵敏度和更少的筛选化合物干扰。
弹性蛋白酶催化弹性蛋白的水解,弹性蛋白是弹性纤维的主要成分,与胶原蛋白一起决定结缔组织的机械性能。弹性蛋白酶具有许多其他天然底物,包括蛋白聚糖、胶原蛋白和纤连蛋白。
Note: Thaw all kit components to room temperature before starting the experiments.
1X Assay buffer: Add 15 mL of 2X assay buffer (Component D) to 15 mL of deionized water
Elastase substrate solution: Dilute elastase substrate (Component A) 100-fold in 1X assay buffer . Prepare fresh substrate solution for each experiment.
Note: Dilute substrate according to the amount needed. Save the rest of the 100x substrate stock solution for future experiments.
Elastase diluents: Dilute the elastase enzyme (Component C) 40-fold in 1X assay buffer . The amount of enzyme is enough for a full 96-well plate. If not using the entire plate, adjust the amount of enzyme to be diluted accordingly.
Note: Prepare elastase diluents immediately before use. Do not vortex the enzyme solution.Prolonged storage or vigorous agitation of the diluted enzyme will cause denaturation. Store the enzyme solution on ice.
Rh110 弹性蛋白酶检测试剂盒使用荧光肽底物检测弹性蛋白酶活性。弹性蛋白酶裂解后,肽释放出具有亮绿色荧光的 Rh110 荧光团,可在 Ex/Em=496 /520 nm 处检测到。荧光强度的增加与酶活性成正比。该试剂盒不需要任何分离步骤,可用于连续测量弹性蛋白酶的动力学。Rh110 的更长波长光谱和更高消光系数提供更高的灵敏度和更少的筛选化合物干扰。
弹性蛋白酶催化弹性蛋白的水解,弹性蛋白是弹性纤维的主要成分,与胶原蛋白一起决定结缔组织的机械性能。弹性蛋白酶具有许多其他天然底物,包括蛋白聚糖、胶原蛋白和纤连蛋白。
Note: Thaw all kit components to room temperature before starting the experiments.
1X Assay buffer: Add 15 mL of 2X assay buffer (Component D) to 15 mL of deionized water
Elastase substrate solution: Dilute elastase substrate (Component A) 100-fold in 1X assay buffer . Prepare fresh substrate solution for each experiment.
Note: Dilute substrate according to the amount needed. Save the rest of the 100x substrate stock solution for future experiments.
Elastase diluents: Dilute the elastase enzyme (Component C) 40-fold in 1X assay buffer . The amount of enzyme is enough for a full 96-well plate. If not using the entire plate, adjust the amount of enzyme to be diluted accordingly.
Note: Prepare elastase diluents immediately before use. Do not vortex the enzyme solution.Prolonged storage or vigorous agitation of the diluted enzyme will cause denaturation. Store the enzyme solution on ice.
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Rh110 弹性蛋白酶检测试剂盒 荧光法
¥4004









