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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
APC anti-mouse CD45.2 Antibody
- CAS号：
APC anti-mouse CD45.2 Antibody
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
25 ug/ 100 ug
| 规格： | 25 ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥2100.0 |
APC 抗小鼠 CD45.2 抗体、APC anti-mouse CD45.2 Antibody详细如下：
Description：
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names：Ly-5.2, LCA
Isotype：Mouse (SJL) IgG2a, κ
Isotype Control：APC Mouse IgG2a, κ Isotype Ctrl (FC)
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：B10.S mouse thymocytes and splenocytes
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration：0.2 mg/ml
Storage & Handling：The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：FC - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser：Red Laser (633 nm)
Application Notes：
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References：
1.Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2.Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3.Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4.Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5.Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6.Pascal V, et al. 2007. J. Immunol. 179:1751.
7.Burman AC, et al. 2007. Blood 110:1064.
8.Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9.Phan TG, et al. 2007. Nature Immunol. 8:992.
10.Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11.Wen T, et al. 2013. PNAS. 110:6067. PubMed
12.Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1.Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
2.Markey K, et al. 2014. J Immunol. 192:5426. PubMed
3.Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
4.Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
5.Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
6.Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
7.Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
8.Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
9.Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
10.Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
11.Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
12.Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
Structure：Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution：
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function：Phosphatase, T and B cell activation
Ligand/Receptor：Galectin-1, CD2, CD3, CD4
Biology Area：Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family：CD Molecules
Antigen References：
1. Suzuki K, et al. 2000. Immunity 13:691.
Description：
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names：Ly-5.2, LCA
Isotype：Mouse (SJL) IgG2a, κ
Isotype Control：APC Mouse IgG2a, κ Isotype Ctrl (FC)
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：B10.S mouse thymocytes and splenocytes
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration：0.2 mg/ml
Storage & Handling：The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：FC - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser：Red Laser (633 nm)
Application Notes：
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References：
1.Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2.Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3.Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4.Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5.Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6.Pascal V, et al. 2007. J. Immunol. 179:1751.
7.Burman AC, et al. 2007. Blood 110:1064.
8.Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9.Phan TG, et al. 2007. Nature Immunol. 8:992.
10.Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11.Wen T, et al. 2013. PNAS. 110:6067. PubMed
12.Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1.Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
2.Markey K, et al. 2014. J Immunol. 192:5426. PubMed
3.Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
4.Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
5.Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
6.Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
7.Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
8.Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
9.Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
10.Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
11.Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
12.Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
Structure：Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution：
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function：Phosphatase, T and B cell activation
Ligand/Receptor：Galectin-1, CD2, CD3, CD4
Biology Area：Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family：CD Molecules
Antigen References：
1. Suzuki K, et al. 2000. Immunity 13:691.
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文献和实验相关实验
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
，发挥各种免疫功能；后者则是构成B细胞表面的抗原受体。我们常说的抗体其实就是分泌型的Ig。 抗体的产生机制 图1：抗体产生机制示意图 B淋巴细胞通过B细胞受体（BCR）识别抗原，抗原提呈细胞（APC）对抗原进行摄取、加工、递呈，辅助性T细胞（Th）被APC激活后分泌多种细胞因子与B细胞上的细胞因子受体结合并相互作用向B细胞提供协同刺激信号。一旦受到刺激，B细胞开始分裂，最终转化为可分泌抗原特异性抗体的浆细胞。 抗体的基本结构 所有Ig的单体结构非常类似，由两条重链和两条轻链组成，重链之间
出现IgG 分子50 and 25 kDa 的重链和轻链条带。 5 二抗的选择 二抗应选用与使用的一抗相同的物种来源，例如：如果你的一抗是小鼠的单克隆抗体，二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用， 二抗一般连接荧光素FITC 或发光团。 6 双重染色抗体的选择用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一，二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 想了解
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APC 抗小鼠 CD45.2 抗体
￥600 - 2100
