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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
APC/Cyanine7 anti-mouse CD45.2 Antibody
- CAS号：
APC/Cyanine7 anti-mouse CD45.2 Antibody
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
25 ug/ 100 ug
| 规格： | 25 ug | 产品价格： | ￥2100.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥5300.0 |
APC/Cyanine7 抗小鼠 CD45.2 抗体、APC/Cyanine7 anti-mouse CD45.2 Antibody详细如下：
Description：
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names：Ly-5.2, LCA
Isotype：Mouse (SJL) IgG2a, κ
Isotype Control：APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：B10.S mouse thymocytes and splenocytes
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration：0.2 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：FC - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser：Red Laser (633 nm)
Application Notes：
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References：
1.Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2.Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3.Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4.Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5.Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6.Pascal V, et al. 2007. J. Immunol. 179:1751.
7.Burman AC, et al. 2007. Blood 110:1064.
8.Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9.Phan TG, et al. 2007. Nature Immunol. 8:992.
10.Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11.Wen T, et al. 2013. PNAS. 110:6067. PubMed
12.Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1.Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed
2.Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
3.White C, et al. 2015. J Immunol. 194:697. PubMed
4.Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
5.Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
6.Li J, et al. 2020. Elife. 9:00. PubMed
7.Laubreton D, et al. 2020. Viruses. 12:00. PubMed
8.Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
9.Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
10.Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
11.Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed
12.Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
See More：
StructureProtein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution：
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function：Phosphatase, T and B cell activation
Ligand/Receptor：Galectin-1, CD2, CD3, CD4
Biology Area：Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family：CD Molecules
Antigen References：
1. Suzuki K, et al. 2000. Immunity 13:691.
Description：
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names：Ly-5.2, LCA
Isotype：Mouse (SJL) IgG2a, κ
Isotype Control：APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：B10.S mouse thymocytes and splenocytes
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration：0.2 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：FC - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser：Red Laser (633 nm)
Application Notes：
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References：
1.Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2.Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3.Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4.Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5.Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6.Pascal V, et al. 2007. J. Immunol. 179:1751.
7.Burman AC, et al. 2007. Blood 110:1064.
8.Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9.Phan TG, et al. 2007. Nature Immunol. 8:992.
10.Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11.Wen T, et al. 2013. PNAS. 110:6067. PubMed
12.Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1.Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed
2.Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
3.White C, et al. 2015. J Immunol. 194:697. PubMed
4.Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
5.Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
6.Li J, et al. 2020. Elife. 9:00. PubMed
7.Laubreton D, et al. 2020. Viruses. 12:00. PubMed
8.Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
9.Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
10.Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
11.Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed
12.Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
See More：
StructureProtein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution：
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function：Phosphatase, T and B cell activation
Ligand/Receptor：Galectin-1, CD2, CD3, CD4
Biology Area：Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family：CD Molecules
Antigen References：
1. Suzuki K, et al. 2000. Immunity 13:691.
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APC/Cyanine7 抗小鼠 CD45.2 抗体
￥2100 - 5300
