APC/Cyanine7 抗小鼠 CD45.2 抗体、APC/Cyanine7 anti-mouse CD45.2 Antibody详细如下: Description: CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. Other Names:Ly-5.2, LCA Isotype:Mouse (SJL) IgG2a, κ Isotype Control:APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl Reactivity:Mouse Antibody Type:Monoclonal Host Species:Mouse Immunogen:B10.S mouse thymocytes and splenocytes Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation:The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions. Concentration:0.2 mg/ml Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application:FC - Quality tested Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Excitation Laser:Red Laser (633 nm) Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. Application References: 1.Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) 2.Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) 3.Suzuki K, et al. 2000. Immunity 13:691. (IHC) 4.Shen FW, et al. 1986. Immunogenetics 24:146. (IP) 5.Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. 6.Pascal V, et al. 2007. J. Immunol. 179:1751. 7.Burman AC, et al. 2007. Blood 110:1064. 8.Kincaid EZ, et al. 2007. J. Immunol. 179:3187. 9.Phan TG, et al. 2007. Nature Immunol. 8:992. 10.Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed 11.Wen T, et al. 2013. PNAS. 110:6067. PubMed 12.Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed Product Citations: 1.Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed 2.Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed 3.White C, et al. 2015. J Immunol. 194:697. PubMed 4.Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed 5.Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed 6.Li J, et al. 2020. Elife. 9:00. PubMed 7.Laubreton D, et al. 2020. Viruses. 12:00. PubMed 8.Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed 9.Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed 10.Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed 11.Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed 12.Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed See More: StructureProtein tyrosine phosphatase (PTP) family, 180-240 kD Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice Function:Phosphatase, T and B cell activation Ligand/Receptor:Galectin-1, CD2, CD3, CD4 Biology Area:Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers Molecular Family:CD Molecules Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.