DKK-2 Fc human 融合蛋白

Product Sheet

DKK-1 Fc fusion, human recombinant
Catalog # CB031-0471、CB031-0472、CB031-0473
Description DKK-1, Dickkopf-related protein-1, is a member of the DKK protein family which includes DKK-1 through DDK-4. DKK was originally identified as a head-forming molecule in Xenopus. Mechanistic studies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway by forming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling is essential for posterior patterning and anterior development in vertebrates, supported by the discovery that DKK-1 knockout mice lack head formation.When DKK-1 is fused to the Fc fragment of human IgG1. This fusion renders stability of the protein in vitro and in vivo without significant loss of activity. ExCell expresses DKK-1 Fc fusion in human 293 cells and purifies it by protein A column.
Formulation Lyophilized in sterile filtered solution of PBS.
Reconstitution Before reconstitution, a brief spin is recommend to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a desired concentration.
Stability The lyophilized protein is stable for at least one year if stored at -80 degreeC. Reconstituted protein is stable for at least four weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
Purity Greater than 90% as determined by SDS-PAGE analysis
Biological Activity The activity was determined by using a TCF reporter gene assay in cultured human cells. The IC50 ranges from 200 - 1000 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. Activity in other assays should be determined by each individual setting.
Country of Origin China
For Research Use Only. Not for Use in Human.