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Product Sheet
Polyclonal antibodies (rabbit), WNT-3aCatalog #:CB031-0441
Preparation : Immunogen: recombinant human WNT-3a purified from E. coli. Anti-HumanWNT-3a specific antibodies were purified from sera of rabbits by affinitychromatography employing immobilized human WNT-3a matrix.
Source :Rabbits
Formulation : A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Stability :The lyophilized antibody is stable for at least 2 years from date of receipt at -20℃. The reconstituted antibody is stable for at least two weeks at 2-8℃.Frozen aliquots are stable for at least 6 months when stored at -200C. Avoidrepeated freeze-thaw cycles.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to aconcentration of 0.1-1.0 mg/ml.
Applications :Western blot, IHCWestern Blot To detect hWNT-3a by Western Blot analysis, this antibody can be used at aconcentration of 0.1 - 1 μg/ml. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant hWNT-3a is 1 –10ng/lane.
Neutralization Data Not Available.
Cross-reactivityHuman; mouse
Country ofOrigin:China
For Research Use Only. Not for Use in Human.
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文献和实验to 50. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Rabbits: Rabbits are immunized essentially as described above. Injections: First in Freund''s
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
Production of Polyclonal Antibodies in Rabbits
The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera
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