Basic FGF (FGF-2), human recombinant Catalog # CB031-0011、CB031-0012、CB031-0013、CB031-0014
Description The human Basic Fibroblast Growth Factor (bFGF) or FGF-2 is a growth factor important to maintaining pluripotency of many types of stem cells,as well as several other cellular processes such as proliferation.ExCell produces bFGF in Escherichia coli as a single chain, nonglycosylated,polypeptide containing 155 amino acids with a molecular mass of 17.2 kDa. This 155 amino-acid form of bFGF retains full biological activity.This product is suitable for tissue culture uses.
Source Escherichia coli. Endotoxin level of this product is lower than 0.1 EU/ug.
Formulation Lyophilized from sterile filtered PBS solution, carrier-free.
Reconstitution Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product in sterile water to no less than 100ug/ml. Further dilutions should be made in sterile buffer or medium
containing carrier proteins, such as albumin or serum.
Stability The lyophilized protein is stable for at least 1 year if stored at -80 degree C.Reconstituted protein is stable for at least 2 week at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze/thaw.
Purity Greater than 95% by SDS-PAGE analysis
Biological Activity The activity was determined by proliferation of the NIH3T3 cells. The EC50 ranges from 0.1 – 1.0 ng/ml.
For Research Use Only. Not for Use in Humans.