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- Leading Biology
- 美国
- PM45094M5
- 2025年07月09日
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- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
20 ug
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Abstract Table of Contents Materials Figures Literature Cited Abstract Protein microarrays
(9 ). Because its systemic administration was shown to mediate the regression of some mouse tumors (10 ), TNF-α has attracted much attention as a potential antitumor reagent (11 ). However, the problem of its dose-dependent toxicity has been particularly apparent in human
with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
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