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- ￥2130
- Leading Biology
- AMRa00060V
- 美国
- 2025年07月11日
- IHC 1:150
- Rat
- Human
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- 文献和实验
- 技术资料
- 靶点：
BrdU
- 浓度：
1 mg/ml
- 应用范围：
IHC 1:150
- 宿主：
Rat
- 适应物种：
Human
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 标记物：
Unconjugated
- 克隆性：
Monoclonal Antibody
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态：
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
- 亚型：
IgG2b
- 免疫原：
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
- 规格：
100 ul
产品概述：BrdU rat monoclonal antibody, clone LBI3B2
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
/Western blotting assays. Another factor important in achieving optimal results is the degree of sensitivity of cells to the antibody-induced apoptosis. As an example, CD95 (Fas)-induced apoptosis of human Jurkat cell line by monoclonal antibody EOS
Single-molecule analysis of DNA replication by molecular combing
is then detected with a monoclonal antibody directed against BrdU and DNA fibers are counterstained with an antibody against single-stranded DNA. Replicating DNA fibers are revealed with fluorescent secondary antibodies and are imaged with an epifluorescence
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