- ￥1433
- Merck-Calbiochem
- 203728-50ML
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
Merck
- 英文名：
BRIJ 35 Detergent PROTEIN Gr. 10% Soln
- 规格：
50ML
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文献和实验UV Absorbance (280 nm) Protein Determination
used as a protein standard; 1 mg/mL has an A 280 of 0.66. At low concentrations, protein can be lost from solution by adsorption on the cuvette; the high ionic strength helps to prevent this. Inclusion of a nonionic detergent (0.01% Brij 35
587 µl 662 µl 735 µl TEMED 5.45 µl 4.91 µl 4.64 µl 4.09 µl 3.55 µl 3 µl 10% APS 54.5 µl 49.1 µl 46.4 µl 40.9 µl 35.5 µl 30 µl Sample Gel Solutions Acrylamide concentration of sample gel solution (%) b Stock
Purification of recombinant sBRF M166L
Renaturation of BRF protein Thaw Ni-NTA purified samples. Dilute the sample to a concentration of 0.25 to 0.5mg/ml in the elution buffer. To the BRF sample, add 10% Brij58 detergent to a final concentration of 0.1% Brij, add DTT to 5 mM final concentration
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