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- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
cAMPS 8-Bromo- Sp-Isomer Sodium Salt
- 供应商:
Merck
- 规格:
5UMO
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
Purification Scheme for Ubiquitin Expression Lysate
, since many of the bacterial proteins will fall out of solution. Centrifuge the solution at 10,000 RPM in a Sorvall SS-34 rotor for 10 minutes at 4o C. Then adjust the pH with sodium hydroxide so that it is 5.1. This prevents other proteins from precipitating during your ion
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
methods developed to examine long-range chromosomal interactions in vivo, see Simonis et al. (2007). For additional details on the 6C method presented here, see Tiwari et al. (2008). MATERIALS Reagents 5-Bromo-4-chloro-3-indoyl-β-D
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