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- 文献和实验
- 技术资料
- 供应商:
Merck
- 库存:
大量
- 英文名:
cAMPS 8-Bromo- Rp-Isomer Sodium Salt
- 规格:
5UMO
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
methods developed to examine long-range chromosomal interactions in vivo, see Simonis et al. (2007). For additional details on the 6C method presented here, see Tiwari et al. (2008). MATERIALS Reagents 5-Bromo-4-chloro-3-indoyl-β-D
M Tris pH 9.6 200 µl 1M MgCl2 500 µl 5 mg/ml BCIP in dimethyl formamide (BCIP= 5-bromo-4- chloro-3-indoyl phosphate, toluidine salt), US Biochemical). Store this stock at -20° wrapped in foil; it's light sensitive. 2.5 ml 2 mg/ml NBT (p-nitro blue
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