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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
HA; HA tag; HA-tag
- 浓度:
Affinity purification
- 应用范围:
WB (Mus musculus) IP (Mus musculus)
- 宿主:
Mouse
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 保存条件:
Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
形式:Liquid 储存溶液:Buffer: PBS with 50% glycerol, pH7.3.
- 亚型:
IgG
- 免疫原:
A synthetic peptide corresponding to HA tag.
- 规格:
50 µl/100 µl
| 规格: | 50 µl | 产品价格: | ¥1095.0 |
|---|---|---|---|
| 规格: | 100 µl | 产品价格: | ¥1890.0 |
稀释方法:WB 1:500 - 1:5000
总结:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.
Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
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文献和实验suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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