Background: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
Immunogen: Recombinant full-length protein of active Rheb.
Applications: IP, IHC, IF
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgM
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles
Immunoprecipitation/Western blot:
Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. TD-26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. ).