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- 2025年07月10日
- WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
优利科（上海）生命科学有限公司
- 库存：
100
- 靶点：
详询
- 级别：
科研
- 目录编号：
/
- 克隆性：
多克隆
- 抗原来源：
详见说明书
- 保质期：
1年
- 抗体英文名：
Anti-RanBP7/Importin 7
- 抗体名：
RanBP7/Importin 7抗体
- 标记物：
详询
- 宿主：
兔
- 适应物种：
/
- 免疫原：
KLH conjugated synthetic peptide derived from human RanBP7/Importin 7
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 保存条件：
-20℃
- 浓度：
1mg/ml
- 规格：
详询
| 英文名称 Anti-RanBP7/Importin 7 |
| 中文名称 RAN结合蛋白7抗体 |
| 别 名 Imp 7; Imp7; Importin-7; Importin7; IPO 7; IPO7; IPO7_HUMAN; Ran binding protein 7; Ran-binding protein 7; RANBP 7; RanBP7. |
| 浓 度 1mg/1ml |
| 规 格 0.2ml/200μg |
| 抗体来源 Rabbit |
| 克隆类型 polyclonal |
| 交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep |
| 产品类型 一抗 |
研究领域 细胞生物 免疫学 信号转导 转录调节因子 表观遗传学 |
| 蛋白分子量 predicted molecular weight: 119kDa |
| 性 状 Lyophilized or Liquid |
| 免 疫 原 KLH conjugated synthetic peptide derived from human RanBP7/Importin 7 |
| 亚 型 IgG |
| 纯化方法 affinity purified by Protein A |
| 储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| （石蜡切片需做抗原修复） |
| not yet tested in other applications. |
| optimal dilutions/concentrations should be determined by the end user. |
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.  |
| Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 Importin 7 is a 1,038 amino acid protein encoded by the human gene IPO7. Importin-7 belongs to the importin b family and contains one importin N-terminal domain. Importin-7 functions in nuclear protein import, either by acting as an autonomous nuclear transport receptor or as an adapter-like protein in association with the Importin b subunit KPNB1. Acting autonomously, Importin-7 is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to Importin-7, the Importin-7/substrate complex dissociates and Importin-7 is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. Importin-7 is a nuclear protein that is expressed in most tissues. |
| Function : Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. |
| Subunit : Interacts with H2A, H2B, H3 and H4 histones (By similarity). Forms a heterodimer with KPNB1. Interacts with KPNB1, SNUPN, XPO1, RPL23A, RPS7, RPL5 and HIV-1 reverse transcription complex integrase. Binds directly to nuclear pore complexes (By similarity). Interacts with H2A, H2B, H3 and H4 histones. Binds to HIV-1 Rev. |
| Subcellular Location : Cytoplasm. Nucleus. |
| Similarity : Belongs to the importin beta family. |
| Contains 1 importin N-terminal domain. |
Database links : UniProtKB/Swiss-Prot: O95373.1 |
| Anti-Smad2/3(Mothers against decapentaplegic homolog 2) Smad 2/3抗体 |
| Anti-Smad4(Mothers against decapentaplegic homolog 4) Smad4抗体 |
| Anti-Smad7(Mothers against decapentaplegic homolog 7) Smad 7抗体 |
| Anti-SM-MHC（Cardiac myosin heavy chain） 心肌肌凝蛋白抗体 |
| Anti-Snake poison Protein 抗蛇毒蛋白抗体（蝮蛇） |
| Anti-Snake poison Protein 抗蛇毒蛋白抗体（中华眼镜蛇） |
| Anti-SNAP-25 (synaptosomal-associated protein 25) 突触相关膜蛋白25抗体 |
| Anti-Socs 1 (suppressor of cytokine signaling 1) 细胞因子信号传导抑制蛋白1抗体 |
| Anti-Socs 3 (suppressor of cytokine signaling 3) 细胞因子信号传导抑制蛋白3抗体 |
| Anti-SOD1 (superoxide-dimutase-1) 超氧化物歧化酶1抗体 |
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文献和实验的，但是这结果是不是假阳性呢？还得做个阴性对照。阴性对照如何设置呢？考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y（co-IP）： 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白（比如，抗 X 抗体是 Mouse IgG，阴性对照可使用 Mouse 免疫球蛋白） beads 对蛋白的非特异结合* 同上，此外还可进行 Pre clear，IP 正式实验前排除非特异结合蛋白。如果使用琼脂
一、原理： 免疫共沉淀（co-immunoprecipitation，Co-IP）技术是检测蛋白质间相互作用的经典方法,基本原理是以细胞内源性靶蛋白为诱饵，将靶蛋白抗体与细胞总蛋白进行共孵育，促进免疫复合物的形成；随后加入能够与抗体Fc段结合的proreinA/G（预先结合固化在琼脂糖小珠上），形成“结合蛋白-靶蛋白-靶蛋白抗体-proteinA/G小珠”复合物，纯化该复合物后凝胶电泳分离蛋白，应用Westernblot或者质谱技术鉴定靶蛋白的结合蛋白。 与其它研究方法相比，免疫
染色体免疫共沉淀（Chromatin immunoprecipitation，ChIP）方法程序
），剪切后的DNA小片段与结合蛋白结合（Isolate the chromatin. Shear DNA along with bound proteins into small fragments.） 第三步：用特异性 抗体 与DAN结合蛋白结合，用沉淀法分离复合体。反向交联操作释放出DNA，并消化蛋白质（Bind antibodies specific to the DNA-binding protein to isolate the complex
