相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Millipore 0.45 PVDF膜(6.6×8.5cm)
- 供应商:
上海博尔森生物科技有限公司
- 保存条件:
RT
- 规格:
10张/包
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验:millipore PVDF膜 0.45 um 转膜方式(恒压、恒流):湿转,恒流0.3A。 转膜时间:180min 设备:“Bio-Rad ” 建议:电转保持电压在70 V以上,保持低温,如果电压低过70就需要换缓冲液了。 蛋白来源:白血病细胞
用1×PBS稀释的包被抗体,即可完成抗体的包被。1、实验卡片填写:设计好试验,把每个孔要加入的内容做成卡片,到时候就会从容很多。2、每孔加入15μLMagi™ Coating Buffer预湿。注意:(1)未润湿PVDF膜是洁白、不透明的,经Magi™ Coating Buffer润湿后,颜色变亮,呈半透明状,很容易观察二者的区别。(2)加Magi™ Coating Buffer时,枪头应该靠在孔壁接近孔底的地方,注意枪头不要刺到PVDF膜。(3)若加入的Magi™ Coating Buffer挂
。但不适合荧光。PVDF膜特别注意的是需要100%甲醇预处理(不超过15秒)再用缓冲液平衡,才能用,而且适用过程中万一干了也要同样程序再处理(不过要真出现这种问题也是自己欠扁,谁要你不小心呢,转膜前处理也就罢了,转膜后再这样处理可能会影响后继的抗体识别呢)。PVDF膜同样分0.45um和0.2um的,后者孔径小,对小分子蛋白有较好的拦截吸附,背景可能会比前者稍高。 pvdf膜生产商: MILLIPORE 特价 常年 现货 IPVH00010 0.45
技术资料暂无技术资料 索取技术资料









