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50个即用型裂解管,包含0.5mm玻璃珠
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文献和实验花粉管染色-Aniline Blue Staining of Pollen/Pollen Tubes
Aniline Blue Staining of Pollen/Pollen Tubes花粉管染色 Materials Fixative: 10% Acetic Acid in ethanol 1M NaOH 50 mM KPO4 buffer, pH 7.5: 4.17 mL 1M K2 HPO4 0.83 mL 1M KH2 PO4 0.01% aniline
Aninexpen sivealter native to glass milk for DNA purificatio
) binds 3 -4.5μl of DNA. 4. Spin for 1 min in a microcentrifuge. Discard the supernatant. 5. Pulse spin, and carefully remove residual liquid. 6. Suspend the pellet in 500μl of 50 mM NaCl, 10 mM TrisHCl pH 7.5, 2.5 mM EDTA, 50
Yeast mini whole cell extract using glass beads for western analysis
in 20 ml cold extraction buffer (containing DTT and protease inhibitors).Keep everything cold from this point on. 3.Resuspend cells in 600 microliters extraction buffer and transfer to 13 x100 mm glass test tubes on ice.Add 400 microliters glass beads
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