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文献和实验GLASS BEADS 0.45 - 0.5mm beads (Sigma) Clean by soaking in nitric acid -->stir bar, O/N.** **Note, dispose of acid in appropriate flask. Wash w/ lots distilled water
Yeast mini whole cell extract using glass beads for western analysis
in 20 ml cold extraction buffer (containing DTT and protease inhibitors).Keep everything cold from this point on. 3.Resuspend cells in 600 microliters extraction buffer and transfer to 13 x100 mm glass test tubes on ice.Add 400 microliters glass beads
Purification of DNA Fragments from Agarose Gels Using Glass Beads
to glass surfaces at high salt concentration. A very high DNA binding capacity is achieved by using a fine powder of crushed glass. After washing, the DNA can be readily eluted from the glass in water, at a final concentration of up to 100 �g/mL.
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