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- 禾午生物
- 国产
- P39131
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20℃&负80℃
- 保质期：
3个月~12个月
- 英文名：
pLV2-TRP53(mouse)-R172H-FLAG-PGK-Puro
- 库存：
99
- 供应商：
禾午生物
- 规格：
干粉&甘油菌
| 别称 | NM_011640.3;bbl; bfy; bhy; p44; p53; Tp53 |
| 启动子 | CMV |
| 复制子 | pUC |
| 克隆菌株 | Stbl3 |
| 培养条件 | 37度 |
| 质粒宿主 | 哺乳细胞,慢病毒 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| 筛选标记 | Puro |
| pLV2-CMV-TERT(human)-IRES-Puro |
| pLV3-CMV-Fluc-MCS-EF1a-Puro |
| pLV2-CMV-Myc-HAT1(human)-PGK-Puro |
| pLV3-CMV-MCS-EF1a-mCherry-Puro |
| pLV2-CMV-TSPAN4(mouse)-EGFP-PGK-Puro |
| pLV3-CMV-TGFBI(human)-EF1a-Puro |
| pLV2-TRP53(mouse)-R270H-FLAG-PGK-Puro |
| pLV2-TRP53(mouse)-R172H-FLAG-PGK-Puro |
| pLV2-CMV-HA-TURBOID(ecoli)-V5-Puro |
| pLV3-CMV-HRCT1(human)-EF1a-Puro |
| pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP |
| pLV2-CMV-WNT5B(pig)(1同义突变)-FLAG-IRES-EGFP-Puro |
| pHR-PGK-NO1-synNotch-Gal4VP64 |
| pLV3-CMV-METTL16(rat)-3×FLAG-EF1a-CopGFP-Puro |
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文献和实验Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Primary mouse embryonic fibroblasts (PMEFs) have a number of properties that make them an attractive cell culture model. Relative to other primary explant cultures they are easy to establish and maintain, proliferate rapidly and, as a result
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
技术资料