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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20℃&负80℃
- 保质期:
3个月~12个月
- 英文名:
pGEM-T7-UTR(Xenopus)-Slc26a8(mouse)-3×FLAG质粒
- 库存:
99
- 供应商:
禾午生物
- 规格:
干粉&甘油菌
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文献和实验Xenopus Oocyte Microinjection and Ion-Channel Expression
skeletal muscle, mouse kidney, rat spleen, Torpedo electric organ; see Lane, 1983; Colman, 1984; Soreq, 1985 for reviews). The value of the Xenopus oocyte for in vitro translation studies is now recognized by the oocyte’s ability to correctly assemble
a function for mos in meiosis (4) . Loss-of-function studies by microinjection of 5′ ATG spanning antisense oligonucleotides established this point when Xenopus oocytes failed to undergo germinal vesicle stage breakdown and thus failed to complete meiosis
sites. Tuschl, et al. recommend against designing siRNA to the 5' and 3' untranslated regions (UTRs) and regions near the start codon (within 75 bases) as these may be richer in regulatory protein binding sites. UTR-binding proteins and/or translation
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