Recombinant SARS-CoV-2 S Protein RBD (V367F, C-6His)
Catalog #: DRA75 Derived From: Human Cells
Description:
Recombinant SARS-CoV-2 S protein RBD Protein is produced by our Mammalian expression system and the target gene encoding Arg319-Phe541(V367F) is expressed with a 6His tag at the C-terminus.
Accession: QHD43416.1
Names(Known as): SARS-CoV-2 RBD Protein; SARS-CoV-2 Spike RBD Protein
Quality Control:
Mol Mass: 26kDa AP Mol Mass: 35kDa, reducing conditions
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Formulation:
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4
Shipping:
The product is shipped on dry ice pack.
Upon receipt, store it immediately at the temperature listed below.
Storage:
Reconstituted protein solution should be stored at ≤ -20°C.
Purification:
Affinity purification chromatography.
Immunoreactivity:
Antigen was assessed by binding ability with ACE-2(Cat#C05Y) or Anti-SARS-CoV-2-S1 mAb(5D9)(Cat#NC025).
Application:
Immunogen, calibrator or standard.
Background:
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.Some findings indicated that the mutated viruses may be evolved to acquire remarkably increased infectivity.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY