Human TNF alpha (诊断原料,感染类）

Recombinant Human TNFα 

 

Catalog #:  DC008        Derived From: E.coli   

 

Description:

Recombinant Human Tumor Necrosis Factor α/TNFα is produced by E.coli expression system and the target gene encoding Val77-Leu233 is expressed.

Accession: P01375

Names(Known as):Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2

 

Quality Control:

Mol Mass:  17.5kDa     AP Mol Mass:  16kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

 

Formulation:

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.

 

Shipping:

The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Ion-exchange chromatography.

 

Immunoreactivity: 
Confirmed by reaction with monoclonal antibodies specific to TNFα.

 

Application:
Immunogen, calibrator or standard.

 

Background:

TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

 

References:

BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer[J]. Hexiang Wang. et al. Biochemical and Biophysical Research Communications. 2021.

Discovery of bardoxolone derivatives as novel orally active necroptosis inhibitors[J]. Yuanyuan Wang. et al. European Journal of Medicinal Chemistry. 2020.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY