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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
QIAseq FX DNA Library UDI-D Kit (96)
- 库存:
以实际库存为准
- 供应商:
QIAGEN/凯杰
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文献和实验freeshotgun sequencing library from miniprep BAC DNA
extraction kit. In the last step, use 30 μl of EB( elution buffer) to elute DNA from column and collect ~ 28 μl DNA. 11.Ligate Ligate size selected DNA fragments into subvectors using Ready-To -Go TM pUC18 Smal1/BAP Ligase ( Phamarcia Biotech
free shotgun sequencing library from miniprep BAC DNA
with gelase ( Gelase TM Agarose Gel-Digesting Properation, Epicentre Technologies, #09200) or QIAquick Gel Extraction Kit ( Qiagen, # 28704) , or GeneClean Kit for Purification of DNA from agarose gel ( Bio 101, #3105). 9.Sonication and end-repair DNA
entitled "QIA gel Extraction Kit Protocol. Include Step #9 and elute in 1X 30µl EB, and 1X in 30µl dH2O. Note, try to run gel such that DNA is contained within 2-3 gel slices. If DNA extracted from too many gel slices and eluants are combined
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