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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C/-80°C
- 保质期:
12个月
- 英文名:
Recombinant Human Insulin-like growth factor-binding protein 5 protein(IGFBP5)
- 库存:
4
- 供应商:
上海禾午生物科技有限公司
- 规格:
20ug/100ug/1mg
产品详情
纯度:>96% as determined by SDS-PAGE and HPLC.
内毒素:Less than 1.0 EU/μg as determined by LAL method.
生物活性:Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4μg/ml, corresponding to a specific activity of >2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
基因名:IGFBP5
Uniprot No.:P24593
别名:IBP 5; IBP-5; IBP5; IBP5_HUMAN; IGF binding protein 5; IGF BP5; IGF-binding protein 5; IGFBP 5; IGFBP-5; IGFBP5; Insulin like growth factor binding protein 5; Insulin-like growth factor-binding protein 5
种属:Homo sapiens (Human)
蛋白长度:Full Length of Mature Protein
来源:E.Coli
分子量:28.6 kDa
表达区域:21-272aa
蛋白标签:Tag-Free
产品提供形式:Liquid or Lyophilized powder
功能:igf结合蛋白可延长igf的半衰期,并可抑制或刺激igf在细胞培养中的促生长作用。它们改变了igf与其细胞表面受体的相互作用。
更多活性蛋白请浏览上海禾午生物科技有限公司
上海禾午生物科技有限公司 提供重组蛋白生产服务His、GST、MBP等多种表达方式同时进行,确保获得蛋白
蛋白表达与纯化服务 纯化的蛋白经过ELISA,Western-blot,或者其它生物活性验证标签切除测序报告
上海禾午生物科技有限公司是一家生物高新技术企业,产品覆盖分子生物学、细胞生物学、生物学试剂、标准物质,等领域。以研发、销售为核心致力于生命科学产品。已与全国各大科研院校、医院、企业以及国外一些科研机构有良好的合作关系。
蛋白的保存
纯化的蛋白往往需要在一定的保存条件,以保证目标蛋白的稳定,避免活性丧失。蛋白溶液的保存:1.缓冲液pH避免接近蛋白的等电点;2.根据每次使用的量分装成多管,这样可避免反复冻融;3.加入稳定剂如甘油,DTT, TritonX-100等。具体操作根据实验需要和蛋白特性,多数蛋白都可以加入终浓度为50%的甘油后-80℃保存
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文献和实验Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins that are present in all extracellular fluids (1 ). Similarly, insulin-like growth factor-I (IGF-I) and -II are synthesized by connective tissue cells
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
The cellular retinaldehyde-binding protein (CRALBP) is expressed at high levels in vertebrate visual trssue, where it may serve to modulate the interaction of 11-cis -retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE
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