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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site, which is ideal
Establishment of Stable Transfectant of CHO Lec Cells
. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose
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