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文献和实验Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Human papillomaviruses (HPVs) are known to be etiological agents of cervical cancer and have been found in 99.7% of women with high-grade (HG) cervical intraepithelial neoplasia (CIN) precancer. Testing of high-risk HPV (HR-HPV
Preparation of Carboxypeptidase Y and Its Properties
by the formation of 1 mole of labeled serine per mole of enzyme. Carboxypeptidase Y is also sensitive to metal ions such as Cu 2+ ,Hg 2+ ,Fe 3+ ,Fe 2+ ,Mg 2+ .etc. Therefore, one must take the general precaution of preventing
sure there is a good seal, pressure 75-80 mm Hg for1 hr or more blot membrane dry, cut top right corner wrap in saran wrap and UV irradiate, RNA side down for 2.5 min wash couple min in hot 2X SSPE/0.1% SDS sol'n (microwave 30-45sec, 50% power) air
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