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文献和实验Exposing gels and plates containing radioactive samples to X-ray film
below which nothing can be detected. Flashing reduces this threshold. Detection of 3H. 3H cannot be detected by autoradiography unless you have 1 mCi of it in your band. Because this is almost unheard of, it is essential to impregnate a gel containing 3H
Coherent Raman Microscopy Analysis of Plant Cell Walls
Coherent Raman scattering (CRS) microscopy is a label-free method for chemical imaging, as it offers chemical specificity with orders of magnitude better sensitivity than the state-of-the-art confocal Raman scattering microscopy
1. 探针的标记:(1) 如下设置探针标记的反应体系:待标记探针 (1.75pmol/微升) 2微升T4 Polynucleotide Kinase Buffer (10X) 1微升Nuclease-Free Water 5微升[γ-32P]ATP(3,000Ci/mmol at 10mCi/ml) 1微升T4 Polynucleotide Kinase (5-10u/微升) 1微升总体积 10微升按照上述反应体系依次加入各种试剂,加入同位素后,Vortex混匀,再加入T
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