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上海觅拓生物
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文献和实验A chemokine-driven positive feedback loop organizes lymphoid follicles
, establishing a positive feedback loop that is likely to be important in follicle development and homeostasis. In germinal centres the feedback loop is overridden, with B-cell lymphotoxin 1 2 expression being induced by a mechanism
of annealed oligos into 398 μl of water to dilutethe products 200-fold. 2. Cloning of targeting constructs. Select the appropriate cloning backbone vector to insert theannealed oligos ( see Notes 2 and 4 ). Mix reagents listed below toset up the ligation
Inducible shRNA Lentiviral Vectors
1 Component Sample Positive Control Entry clone (50-150 ng/reaction) 1-7 µl
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