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文献和实验hESC Engineering by Integrase-Mediated Chromosomal Targeting
Bacteriophage recombinases can target specific loci in human embryonic stem cells (hESCs) at high efficiency allowing for long-term expression of transgenes. In this chapter, we describe a retargeting system where phiC31 integrase is used
Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli
. The resulting CRIM expression plasmids are then integrated in single copy into a chromosomal phage attachment site by supplying integrase from a helper plasmid. Such integrants can be used for conditional expression of any target gene in single copy
Marker-Free Chromosomal Expression of Foreign and Native Genes in Escherichia coli
Genetic manipulation of Escherichia coli strains for desired traits is the most applied strain engineering approach in industrial applications. For chromosomal insertion of genes and controlled expression of genomic genes in E. coli
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