TransDux virus transduction re

Determining Relative Transduction of Adherent Cells

of cells expressing GFP is recommended MOI for transduction. 注意事项   1. Day 0    1) Include a non-transduced negative control well, where no virus is added. 2. Day 3    1) Make sure to allow

Lentiviral Transduction Protocol for Human Embryonic Stem Cells

Add the desired amount of virus particles to the culture medium. NOTE: For the initial transduction of hESC lines, Dr. Cohen’s lab uses 10 µl of 1x106 TU/ml viral particles for H1 and H9 hESC lines. Since an exact cell count

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

-transduction, which can affect >50% of your cells. This is an indication of high uptake of the virus. We recommend that you continue culturing your cells and proceed with the protocol.         c. Culture the cells for 6 more days, changing the spent