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- 文献和实验
- 技术资料
- 库存:
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- 英文名:
L-Malic Acid Standard Solution (6 g/L)
- CAS号:
97-67-6
- 保质期:
1年
- 供应商:
上海经科化学科技有限公司
- 保存条件:
2-8℃
- 规格:
50mL
RECOMMENDED APPLICATIONS:
Analytical standard solution for use as a control sample or calibrator with analytical test kits that measure L-Malic acid. Especially for use to generate calibration curves for auto-analyser or microplate assay formats with the following enzymatic tests kits: K-LMALAF, K-LMALQR.
| CAS Number: | 97-67-6 |
| Synonyms: | (S)-(-)-Malic acid, (S)-(-)-2-Hydroxysuccinic acid, L-Hydroxybutanedioic acid |
| Molecular Formula: | C4H6O5 |
| Molecular Weight: | 134.08 |
| Concentration: | 6 g/L in 0.02% sodium azide |
| Content: | 50 mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Physical Form: | Solution |
| Stability: | > 5 years under recommended storage conditions |
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文献和实验through the following solutions in this order. i) Chromium trioxide (chromic acid 5%) x6 ii) Distilled water x6 iii) P-L-L solution x1 (double) iv) Absolute methanol x2 4. The slides come off the machine dry
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Standard hybridization technique
sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results is a variation on that described by Church and Gilbert
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