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文献和实验Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
of the ChIP assay is that it gives a researcher the ability to not only detect a specific protein–DNA interaction in vivo, for instance with Pol II, but also to determine the relative density of factors along genes or the entire genome. Though powerful
RNA polymerase II (Pol II) plays a crucial role in eukaryotic biology since it is necessary for the expression of all protein-coding genes as well as most microRNAs and several small nuclear RNAs. Pol II is specifically recruited to core
Site-specific protein-DNA photocrosslinking has proved to be the method of choice for analysis of the formation of nucleoprotein complexes such as those involved in transcription by mammalian RNA polymerase II (RNA Pol II). The method
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