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- C3730
- Invitrogen-MP
- 2025年07月12日
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文献和实验E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
amount of RNA (?2 ug). Add 2 ul of Linear Acrylamide to the mixture. Note: Remember to add 20 ul of 2-mercaptoethanol per 1 ml of QVL Lysis Buffer before use. 3. Add 250ul absolute ethanol (96-100%, room temperature) to the sample and mix thoroughly
【原创】lentivirus calcium phosphate transfection protocl
calcium phosphate transfection protocl： 1. Split 293FT cells about 30% confluence, overnight. 2. Thaw 2*HBS room temperature (not in 37 C). 3. For 10cm dish: 1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
the samples with DEPC treated water. 2. Add 350ul QVL Lysis buffer and mix by vortexing. When process small amount of RNA (?2 ug). Add 2 ul of Linear Acrylamide to the mixture. Note: Remember to add 20 ul of 2-mercaptoethanol per 1 ml of QVL Lysis
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