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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Hyaluronan and proteoglycan link protein 1 (Hapln1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Hyaluronan and proteoglycan link protein 1 (Hapln1)品名简称:
Recombinant Mouse Hapln1 protein货号:
CSB-EP010130MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Hapln1Uniprot No.:
Q9QUP5别名:
Cartilage-linking protein 1;Proteoglycan link protein种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
46.4 kDa表达区域:
10-356aa氨基酸序列:
ISVCWADHHLSDSYTPPDQDRVIHIQAENGPRLLVEAEQAKVFSHRGGNVTLPCKFYRDPTAFGSGIHKIRIKWTKLTSDYLREVDVFVSMGYHKKTYGGYQGRVFLKGGSDNDASLVITDLTLEDYGRYKCEVIEGLEDDTAVVALELQGVVFPYFPRLGRYNLNFHEARQACLDQDAVIASFDQLYDAWRGGLDWCNAGWLSDGSVQYPITKPREPCGGQNTVPGVRNYGFWDKDKSRYDVFCFTSNFNGRFYYLIHPTKLTYDEAVQACLNDGAQIAKVGQIFAAWKLLGYDRCDAGWLADGSVRYPISRPRRRCSPTEAAVRFVGFPDKKHKLYGVYCFRAYN蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix功能2:
/文献:
/研究领域:
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文献和实验Cartilage Proteoglycan Release Assay
contains a number of proteoglycans, the most abundant of which is aggrecan. This proteoglycan is characterized by its association with hyaluronic acid and link protein to form large macromolecular aggregates within the tissue. The large negative charge
of a core protein to which glycosaminoglycans and N - as well as O -linked oligosaccharides are attached. This proteoglycan is able to form specific aggregates with hyaluronic acid and link protein.
Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins
In this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1 ,2 ). The b-PG is derived from cartilage and consists of a trypsin fragment of the proteoglycan core protein
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