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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human FAS-associated death domain protein (FADD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥12372.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2880.0 |
| 规格: | 20ug | 产品价格: | ¥1536.0 |
Research Areas
OthersUniprot ID
Q13158Gene Names
FADDAlternative Name(s)
(FAS-associating death domain-containing protein)(Growth-inhibiting gene 3 protein)(Mediator of receptor induced toxicity)Lead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
1-208aaProtein Length
Full LengthTag Info
C-terminal 6xHis-taggedTarget Protein Sequence
MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEASMW
30.2 kDaPurity
Greater than 85% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
/Function
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文献和实验带有死亡结构域的Fas 结合蛋白 带有死亡结构域的Fas结合蛋白(Fas-associating protein with death domain,FADD)是分子质量为23kDa的胞质蛋白质。人类FADD基因编码区长约2.6kb,有2个外显子,其间被一长约2kb的内含子分隔。外显子1长286bp,编码N端诱导细胞凋亡所必须的死亡效应结构域(deatheffectdomain,DED);外显子2长341bp,编码死亡结构域(DD),此DD基因与Fas
内的死亡域(DD)结合Fas结合蛋白FADD(fas associated protein with DD),FADD再以其N端的死亡效应子域(death effector domain,DED)结合Pro-Caspase-8(或-10),形成一个由Fas-FADD-Pro-Caspase-8(或-10)三种分子组成的复合体,称为诱导死亡的信号复合体(death inducing signaling complex,DISC),其中Pro-Caspase-8(或-10)就可以自身催化
式途径是通过TNF受体家族(如CD95)的受体与配体结合开始的。这些配体有癌症坏死因子(TNF),和其他细胞因子,后者可以由如T淋巴细胞分泌。 在FADD(Fas偶联死亡区域蛋白Fas-associated death domain protein)的协助下,受体不断在细胞质中收集半胱天冬酶原8(Procaspase 8)。后者通过高密度自催化激活自身。活化的半胱天冬酶8(Caspase 8)将会引发所谓的Caspase级联反应。因此在艾滋病人体内,很多未受感染的白血球也会凋亡:HIV
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