sem-5|sem-5蛋白|sem-5蛋白|sem-5 el

Preparing 2-D Protein Extracts from Caenorhabditis elegans

The small nematode Caenorhabditis elegans (C. elegans , 1 mm in length) originally was introduced into the laboratory in 1965 by Brenner (1 ) and has since become an important model organism. Caenorhabditis elegans for a variety of reasons

胶粘剂迁移对层析检测的影响

的玻璃纤维和纤维素膜；2 cm的硝酸纤维素膜）的时间来评定水平移动的速度。使用一种典型的层析检测模式（单克隆抗-ßhCG抗体捕获和胶连，用于结合尿液中的HCG），检测硝酸纤维素膜的性能，蛋白质的应用，捕获线的强度，以及疏水斑块。所有的检测都用两种独立的物料重复检测10次。这些薄片状材料也同时用电镜扫描（SEM）进行分析。对于这些检查，这些薄片状材料的横截面被切出用于粘合层厚度的测量。研究结果图2 不同类型胶粘剂对PuraBind膜的水平迁移的影响。以无薄板材料支撑的膜的迁移检测的结果为对照，该研究

Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis

. Together with microscopy-based genetic screens this method can be used to identify modifiers of protein aggregation and characterize their molecular function. Although described here for analyzing aggregates in C . elegans , NAGE can be adjusted