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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7)品名简称:
Recombinant Caenorhabditis elegans daf-7 protein货号:
CSB-EP311270CXY规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
daf-7Uniprot No.:
P92172别名:
(Abnormal dauer formation protein 7)种属:
Caenorhabditis elegans蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
18.1 kDa表达区域:
235-350aa氨基酸序列:
SHAKPVCNAEAQSKGCCLYDLEIEFEKIGWDWIVAPPRYNAYMCRGDCHYNAHHFNLAETGHSKIMRAAHKVSNPEIGYCCHPTEYDYIKLIYVNRDGRVSIANVNGMIAKKCGCS蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Under harsh environmental conditions, larvae enter a developmentally arrested state known as dauer; TGF-beta-like daf-7 acts to inhibit dauer larva formation and promote growth. May be a ligand to cell surface receptor daf-4. May act as a negative regulator of dauer larva development by transducing chemosensory information from ASI neurons. Involved in sensitivity to CO2 levels. Involved in mate searching behavior of males, acting in concert with the neuropeptide pdf-1. In AWC neurons, acts to promote expression of srsx-3, a member of the GPCR family.功能2:
/文献:
"The homeodomain protein hmbx-1 maintains asymmetric gene expression in adult C. elegans olfactory neurons." Lesch B.J., Bargmann C.I. Genes Dev. 24:1802-1815(2010)研究领域:
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文献和实验Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans
by all stages of animals, and is collectively referred to as dauer pheromone; pheromone concentration is a major trigger for dauer formation. Here, we describe a quantitative dauer formation assay that provides a measure of the potency of single or mixtures
Proteomic Analysis of Caenorhabditis elegans
subjected to two specific stress conditions during development: induction of the dauer state for whole body protein expression and a temperature shift for egg protein expression. Utilizing these differently perturbed C. elegans protein samples
Preparing 2-D Protein Extracts from Caenorhabditis elegans
The small nematode Caenorhabditis elegans (C. elegans , 1 mm in length) originally was introduced into the laboratory in 1965 by Brenner (1 ) and has since become an important model organism. Caenorhabditis elegans for a variety of reasons
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