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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial品名简称:
Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial货号:
CSB-EP311153ALB规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P86268别名:
AnpAFP种属:
Antarctomyces psychrotrophicus蛋白长度:
Partial来源:
E.coli分子量:
17.2 kDa表达区域:
1-20aa(X10S)氨基酸序列:
AGLDLGAASSFGALAFEGVA蛋白标签:
N-terminal 6xHis-KSI-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Antifreeze proteins bind to the surface of ice crystals and inhibit the growth of these crystals, this inhibition causes thermal hysteresis. Causes the shape of ice crystals to change from hexagonal to a bipyramidal shape with rugged facets. Inhibits recrystallization of ice crystals.功能2:
/文献:
/研究领域:
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文献和实验L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白,兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G,也可根据结合力表格进行选择,详见资源信息部分 3、基质—琼脂糖还是磁珠? 琼脂糖 磁珠 优点 简单易用,无需辅助设备 操作简单 孔径大,直径大(50-150
扫描仪中,都采用机械式的二维X,Y线性扫描技术实现,即X,Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置,由于驱动系统的频率限制,驱动器的扫描惯性大,使得扫描效率低,分析时间相当长;并且往复行程长,对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描,我们的实验装置设计如图2,采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描,Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中,对于f-è物镜,满足x=2fè(è为振镜的摆动角度,f为物镜焦距)的线性
质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
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